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how you used this part and how it worked out.
Applications of BBa_K593008
User Reviews
UNIQ4bce64521c2c2eba-partinfo-00000000-QINU
UNIQ4bce64521c2c2eba-partinfo-00000001-QINU
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iGEM Braunschweig 2014
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1. Results of sequencing
After transformation of the BioBrick BBa_K593004 and cultivation in E. coli the plamid DNA was isolated. We determined the nucleotide sequence of the BioBrick BBa_K593008 by use of sequencing techniques. The alignment of BBa_K593008 with the original sequence of mmoC fromMethylococcus capsulatus shows that they differed significantly (see Fig.1).
 Fig. 1 Alignment of BBa_K593008 and the original sequence of mmoC from M. capsulatus. The tested BioBrick does not contain the sequence of mmoC. The DNA sequence is without any distinct function. A further characterization of the BioBrick BBa_K593008 was impossible. We were not able to perform any expression studies or activity tests with this BioBrick.
2. Improvement
Our new BioBrick BBa_K1390002 contains the original sequence of mmoC from Methylococcus capsulatus, as shown in the alignment of our new Brick and the original sequence from Methylococcus capsulatus (see Fig.2).
 Fig. 2 Alignment of BBa_K139002 and the original sequence of mmoC from M. capsulatus. |
